تاثیر ترکیب شیمیایی بر رفتار زینترینگ و خواص ریزساختاری، مکانیکی و بیولوژیکی کامپوزیت‌های هیدروکسی آپاتیت/شیشه بوراتی

نوع مقاله : علمی و پژوهشی

نویسندگان

1 گروه مهندسی مواد و متالورژی، دانشکده مهندسی، دانشگاه فردوسی مشهد، مشهد، ایران.

2 تخصصی مهندسی بافت، گروه علوم تشریح و بیولوژی سلولی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد.

3 گروه علوم تشریح و بیولوژی سلولی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد.

چکیده

در پژوهش حاضر، از روش احتراقی در محلول جهت سنتز ترکیب آمورف بوراتی زیست‌فعال.استفاده شد. آنالیز XRD، FTIR و PSA با هدف مشخصه‌یابی فازی، تشخیص پیوند‌ و تعیین اندازه ذرات انجام شد. از آزمایش‌های MTT و Migration به منظور تعیین زیست‌سازگاری پودرها استفاده شد. پودر سنتز شده دارای ابعاد نانو، ساختار آمورف و تاثیر مثبت بر تکثیر و مهاجرت سلول‌ها است. ترکیب آمورف بوراتی با نانوذرات هیدروکسی آپاتیت به منظور ایجاد داربست‌های کامپوزیتی، در درصدهای وزنی متفاوت ترکیب شده و در دماهای 600، 650 و oC700 به مدت زمان‌های 0.5 و 1 ساعت زینتر شدند. مطالعه الگوی تفرق اشعه ایکس به منظور تشخیص فازها و تست استحکام کششی قطری به منظور سنجش استحکام مکانیکی انجام شد. تصویربرداری FESEM از نمونه‌های شکسته شده در تست استحکام کششی قطری به منظور مشاهده‌ی مسیر ترک، میزان تخلخل و نحوه تاثیر شیشه بوراتی بر زینتر انجام شد. نمونه با wt.% 20 هیدروکسی آپاتیت و wt.% 80 شیشه بوراتی، زینتر شده در oC700 به مدت 1 ساعت، دارای بالاترین استحکام به مقدار 0.02±4.87 است. آنالیز ICP-OES نمونه با wt.% 20 هیدروکسی آپاتیت زینتر شده در oC700 به مدت 1 ساعت بعد از 7 روز غوطه‌وری در مایع شبیه سازی شده‌ی بدن، نشان دهنده افزایش رهایش یون در مقایسه با هیدروکسی آپاتیت خالص زینتر شده است. همچنین کنترل و کاهش رهایش یونی قابل ملاحظه شیشه بوراتی از طریق کامپوزیت و زینتر شدن در مشاهده شد. کامپوزیت ساخته شده دارای قابلیت پیوند با بافت نرم و سخت است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

“The Effect of Chemical Composition on Sintering Process and Microstructural, Mechanical, and Biological Properties of Hydroxyapatite/Borate Glass Composites”

نویسندگان [English]

  • Zeinab Ebrahimi Kahoo 1
  • Negar Akrami 1
  • Mehrnoosh Ghanad 1
  • Simin Nazarnezhad 2
  • Saeid Kargozar 3
  • sahar mollazadeh 1
1 Department of materials and metallurgy engineering/ faculty of engineering/ Ferdowsi university of Mashhad/ Mashhad/ Iran.
2 Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences.
3 Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده [English]

In this study, the solution combustion method was used to synthesize a bioactive borate compound. XRD, FTIR, PSA analyses have been used to identify phase composition, bond determination, and particle size, respectively. In addition, MTT and cell migration assay were utilized to measure the biocompatibility of the particles. The synthesized particles not only were nano in size along with amorphous structure, but also showed positive effects on cell proliferation and migration. To attain bioactive compositional scaffolds, the borate amorphous compound was combined with hydroxyapatite nanoparticles at different weight percentages. Afterward, mixtures were sintered at 600, 650, and 700 oC for 0.5 and 1 h. XRD and DTS analyses were operated to evaluate the phase composition and mechanical strength. FESEM micrograph was evaluated to observe the crack path in addition to porosity and the effect of borate glass on the sintering process. The sample sintered at 700 oC for 1h containing 20% borate glass and 80% of hydroxyapatite, accounted for the highest mechanical strength at 4.87 ± 0.02 MPa. ICP-OES analysis of the sample containing 20% hydroxyapatite, which was sintered at 700 oC for 1 h, showed an increase in ion release compared to pure sintered hydroxyapatite after immersing in SBF for 7 days. Also, ion release of the borate glass has considerably been controlled and diminished by virtue of combining and sintering with hydroxyapatite nanoparticles. Furthermore, the mentioned composition is able to bond with soft tissue as well as hard tissue.

کلیدواژه‌ها [English]

  • hydroxyapatite
  • bioactive glass
  • borate glass
  • sintering
  • scaffold
  • composite
  • nanoparticle
  • tensile strength
  • ion release

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