Synthesis and study of the pH effect on the antibacterial activity of ZnSe nanoparticles against the Escherichia coli and Pseudomonas aeruginosa gram-negative bacteria by using disc-diffusion bioassay

Document Type : Original Articles

Authors

• Dariush Souri ¹
• Saeid Mohaghegh Nejad ²
• Mehdi Ghabooli ³

¹ Faculty of Science, Department of Physics, Malayer University.

² Department of Physics, Malayer University

³ Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University.

10.22067/jmme.2021.58903.0

Abstract

In the present research, ZnSe nanoparticles were synthesized by aqueous method at pHs of 8, 10.2, 11.2 and 12.2 and were grown by microwave irradiation at the times of 0 and 6 minutes. In the synthesis process, Se and Zn ions sources were added to each other at the presence of thioglycolic acid as capping agent . Antibacterial properties of these nanoparticles have been carried out by using disc diffusion method (in solid Muller-Hinton-Agar culture) against the Escherichia coli and Pseudomonas aeruginosa gram-negative bacteria in the loading volume of 140 µL of nanoparticles. Generally, results showed that by increasing the radiation time from 0 to 6 min, bacterial growth inhibition zone increased for Pseudomonas aeruginosa bacteria in compare with Escherichia coli bacteria; the highest antibacterial activity is devoted to the cases of pH=10.2 and 11.2, and these samples can be introduced as new agents in the field of nano-biotechnology applications.

Keywords

• Gram-negative bacteria
• ZnSe nanoparticles
• microwave irradiation
• disc-diffusion method
• antibacterial property

20.1001.1.20087462.1400.33.1.2.6

Main Subjects

• Biomaterials

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