Development of Polycaprolactone/zinc Oxide Nanoparticles/Chitosan Scaffolds for Skin Tissue Engineering and Improving its Mechanical Properties

Document Type : Original Articles

Authors

• Narges Johari ¹
• Fatemezahra Kasaeian ²

¹ Materials Engineering group, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan, Iran.

² Materials Engineering group, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan, Iran

10.22067/jmme.2023.81049.1104

Abstract

Skin, as the largest and one of the most vital organs of the human body, which is subject to damage more than other tissues, consists of three main layers: epidermis, dermis, and hypodermis. Tissue engineering and biomaterials as cell support can be used to regenerate the damaged parts of skin. In the present study, a three-layer scaffold was made for biomimicking the native skin tissue and accelerating wound healing. Poly(ε-caprolactone)/zinc oxide nanoparticles as porous polymer nanocomposite containing 0, 5, 10 and 15 wt.% zinc oxide nanoparticles prepared in two layers using solvent casting/salt leaching method. The third layer of chitosan as the external membrane was added by sodium hydroxide cross-linking agent on the previous two layers. The phase structure, chemical functional group, and morphology of the prepared scaffolds were investigated using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM), respectively. To evaluate the mechanical properties, the prepared scaffolds were subjected to compressive strength test. The obtained results showed that the porosity of the three-layered scaffolds was partially changed in a gradient behavior. The structural integrity, morphological cohesion, and compressive strength of the scaffolds with 5 wt.% zinc oxide nanoparticles in which the cross-linking agent was used to add the chitosan membrane were significantly higher than the other prepared scaffolds.

Keywords

• Scaffold
• Skin tissue engineering
• Poly(&epsilon
• -caprolactone)
• Chitosan
• Zinc oxide nanoparticles
• Cross-linking agent
• Sodium hydroxide

Main Subjects

• Biomaterials

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References

[1] M. Colonna, “Skin function for human CD1a-reactive T cells,”Nature immunology, vol. 11, no. 12, pp. 1079-1080, (2010.).

[2] F. Strodtbeck, “Physiology of wound healing,” Newborn and infant nursing reviews, vol. 1, no. 1, pp. 43-52, (2001).

[3] S. Singh, A. Young, C.E. McNaught, “The physiology of wound healing,” Surgery (Oxford), vol. 35, no. 9, pp. 473-477, (2017).

[4] L.Q. Wu, A.P. Gadre, H. Yi, M.J. Kastantin, G.W. Rubloff, W.E. Bentley, G.F.  Payne, R. Ghodssi, “Voltage-dependent assembly of the polysaccharide chitosan onto an electrode surface,” Langmuir, vol. 18, no. 22, pp. 8620-8625, (2002).

[5] S.K. Misra, S.P., Valappil, I. Roy, A.R. Boccaccini, “Polyhydroxyalkanoate (PHA)/inorganic phase composites for tissue engineering applications,” Biomacromolecules, vol. 7, no. 8, pp. 2249-2258, (2006).

[6] J.P. Vacanti, C.A. Vacanti, “The history and scope of tissue engineering, in Principles of tissue engineering,” Academic Press, (2014).

[7] M. Cheong, I. Zhitomirsky, “Electrodeposition of alginic acid and composite films,” Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 328, no. 1-3, pp. 73-78, (2008).

[8] X. Li, X. Liu, J. Huang, Y. Fan, F.Z. Cui, “Biomedical investigation of CNT based coatings,” Surface and Coatings Technology, vol. 206, no. 4, pp. 759-766, (2011).

[9] N.P. Dhanasekaran, K.S. Muthuvelu, S.K. Arumugasamy, “Recent advancement in biomedical applications of polycaprolactone and polycaprolactone-based materials, Elsevior,” (2022).

[10] M.T. Yen, J.H. Yang, J.L. Mau, “Physicochemical characterization of chitin and chitosan from crab shells,” Carbohydrate polymers, vol. 75, no. 1,  pp. 15-21, (2009).

[11]T. Wang, X.K. Zhu, X.T. Xue, D.Y. Wu, “Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings,” Carbohydrate polymers, vol. 88, no. 1, pp. 75-83, (2012).

[12] I. Corni, M.P. Ryan, A.R. Boccaccini, “Electrophoretic deposition: From traditional ceramics to nanotechnology,” Journal of the European Ceramic Society, vol. 28, no. 7, pp. 1353-1367, (2008).

[13] X. Peng, K. Dong, Y. Zhang, L. Wang, C. Wei, T. Lv, Z.L. Wang, Z. Wu, “Sweat‐Permeable, Biodegradable, Transparent and Self‐powered Chitosan‐Based Electronic Skin with Ultrathin Elastic Gold Nanofibers,” Advanced Functional Materials, vol. 32, no. 20, pp. 2112241, (2022).

[14] T. Yamaguchi, Y. Tanaka, A. Ide-Ektessabi, “Fabrication of hydroxyapatite thin films for biomedical applications using RF magnetron sputtering,” Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, vol. 249, no. 1-2, pp. 723-725, (2006).

[15] J.L. Watson, T. Fang, C.O. Dimkpa, D.W. Britt, J.E. McLean, A. Jacobson, A.J. Anderson, “The phytotoxicity of ZnO nanoparticles on wheat varies with soil properties,” Biometals, vol. 28, no. 1, pp. 101-112, (2015).

[16] M.D. Preda, M.L. Popa, I.A. Neacșu, A.M. Grumezescu, O. Ginghină, “ Antimicrobial Clothing Based on Electrospun Fibers with ZnO Nanoparticles,” International Journal of Molecular Sciences, vol. 24, no. 2, pp. 1629, (2023).

[17] Y.S. Cho, M.W. Hong, S.Y. Kim, S.J. Lee, J.H. Lee, Y.Y. Kim, Y.S. Cho, “Fabrication of dual-pore scaffolds using SLUP (salt leaching using powder) and WNM (wire-network molding) techniques,” Materials Science and Engineering: C, vol. 45, no. 1, pp. 546-555, (2014).

[18] M. Mehrabanian, M. Nasr-Esfahani, “HA/nylon 6, 6 porous scaffolds fabricated by salt-leaching/solvent casting technique: effect of nano-sized filler content on scaffold properties,” International journal of nanomedicine, vol. 6, no. 1, pp. 1651-1659, (2011).

[19] T.S. Singh, P. Singh, R.D.S. Yadava, “Perspectives for Electronic Nose Technology in Green Analytical Chemistry, in Green Polymer Chemistry and Composites,” Apple Academic Press, p.35 (2021).

[20] L. Wang, J. Kang, C. Sun, D. Li, Y. Cao, Z. Jin, “Mapping porous microstructures to yield desired mechanical properties for application in 3D printed bone scaffolds and orthopaedic implants”, Materials & Design, vol. 133, no. 1, pp. 62-68, (2017).

[21] I.S. Suhaimin, S.A. Zubir, T.K. Abdullah, “Effect of leaching agent composition on morphology, thermal and mechanical properties of Bioglass® reinforced polyurethane scaffold,” International Journal of Current Research in Science and Technology, vol. 19, no. 1, pp. 19-27, (2018).

[22] P. Mosallanezhad, H. Nazockdast, Z. Ahmadi, A. Rostami, “Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing,” Frontiers in bioengineering and biotechnology, vol. 10, no. 1, pp. 1027351, (2022).

[23] S.M. Naghib, H.R. Garshasbi, A. Maleki, Y. Zare, K. Rhee, “Smart Stimuli-responsive Biomaterials for Programmed Drug Delivery,” Frontiers in bioengineering and biotechnology, vol. 11, no. 1, pp. 1222034, (2023).

[24] A. De Masi, I. Tonazzini, C. Masciullo, R. Mezzena, F. Chiellini, D. Puppi, M. Cecchini, “Chitosan films for regenerative medicine: Fabrication methods and mechanical characterization of nanostructured chitosan films,” Biophysical Reviews, vol. 11, no. 1, pp. 807-815, (2019).

[25] Z.A. Abdelouhab, D. Djouadi, A. Chelouche, L. Hammiche, T. Touam, “Structural and morphological characterizations of pure and Ce-doped ZnO nanorods hydrothermally synthesized with different caustic bases,” Materials Science-Poland, vol. 38, no. 2, pp. 228-235, (2020).

[26] L. Sun, J. Wang, J. Liang, G. Li, “Boric acid cross-linked 3D polyvinyl alcohol gel beads by NaOH-titration method as a suitable biomass immobilization matrix,” Journal of Polymers and the Environment, vol. 28, no. 1, pp. 532-541, (2020).

[27] K. Santiago-Castillo, D. Del Angel-López, A.M. Torres-Huerta, M.A. Domínguez-Crespo, D. Palma-Ramírez, H. Willcock, S.B. Brachetti-Sibaja, “Effect on the processability, structure and mechanical properties of highly dispersed in situ ZnO: CS nanoparticles into PVA electrospun fibers,” Journal of Materials Research and Technology, vol. 11, no. 1, pp. 929-945, (2021).

[28] S. Mallakpour, N. Nouruzi, “Effect of modified ZnO nanoparticles with biosafe molecule on the morphology and physiochemical properties of novel polycaprolactone nanocomposites,” Polymer, vol. 89, no. 1, pp. 94-101, (2016).

[29] M. Borjigin, C. Eskridge, R. Niamat, B. Strouse, P. Bialk, E.B. Kmiec, “Electrospun fiber membranes enable proliferation of genetically modified cells,” International journal of nanomedicine, Vol. 8, no. 1, pp. 855-864, (2013).

[30] S.B. Aziz, M.H. Hamsan, W.O. Karim, M.F.Z. Kadir, M.A. Brza, O.G. Abdullah, “High proton conducting polymer blend electrolytes based on chitosan: Dextran with constant specific capacitance and energy density,” Biomolecules, vol. 9, no. 7, pp. 267-289, (2019).

[31] R. Augustine, H.N. Malik, D.K. Singhal, A. Mukherjee, D. Malakar, N. Kalarikkal, S. Thomas, “Electrospun polycaprolactone/ZnO nanocomposite membranes as biomaterials with antibacterial and cell adhesion properties,” Journal of Polymer Research, vol. 21, no. 1, pp. 1-17, (2014).

[32] M.M. Rahman, M.S. Islam, G.S. Li, “Development of PLA/CS/ZnO nanocomposites and optimization its mechanical, thermal and water absorption properties,” Polymer Testing, vol. 68, no. 1, pp. 302-308, (2018).

[33] H. Zhu, J. Shen, X. Feng, H. Zhang, Y. Guo, J. Chen, “Fabrication and characterization of bioactive silk fibroin/wollastonite composite scaffolds,” Materials Science and Engineering: C, vol. 30, no. 1, pp. 132-140, (2010).

[34] A.R. Boccaccini, J.A. Roelher, L.L. Hench, V. Maquet, R. Jérǒme, “A composites approach to tissue engineering", 26th Annual Conference on Composites, Advanced Ceramics, Materials, and Structures: B: Ceramic Engineering and Science Proceedings, Wiley Online Library, (2002).

[35] L. Liu, Y. Zhang, C. Li, J. Cao, E. He, X. Wu, F. Wang, L. Wang, “Facile preparation PCL/modified nano ZnO organic-inorganic composite and its application in antibacterial materials,” Journal of Polymer Research, vol. 27, no. 1, pp. 1-11, (2020).