داربست‌های کامپوزیتی ژلاتین-کلسیم‌فسفات: از فرآیند ساخت تا بررسی رفتار مکانیکی و زیستی

نوع مقاله : علمی و پژوهشی

نویسندگان

1 گروه متالورژی و مواد، دانشگاه فردوسی مشهد، مشهد، ایران

2 دکترای حرفه‌ای داروسازی دانشگاه علوم پزشکی مشهد

3 گروه مهندسی متالورژی و مواد دانشگاه فردوسی مشهد، ایران.

4 گروه فارماکولوژی و سم‌شناسی ، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد

چکیده

هدف پژوهش حاضر، ساخت داربست‌های کامپوزیتی از طریق فرآیند انحلالی و خشکایش سرمایشی می‌باشد. اولین مرحله، فرآوری ذرات کلسیم‌فسفات به روش سل‌ژل و سپس، عملیات حرارتی ذرات در دمای ˚C1100 است. طبق نتایج آنالیز تعیین اندازه ذرات و پیکنومتری، اندازه ذرات و دانسیته آن‌ها به ترتیب برابر با nm5±37 و g/cm306/4 می‌باشد. همچنین طبق نتایج حاصل از آنالیز پراش پرتو ایکس (XRD) و آنالیز حرارتی افتراقی (DTA-TG)، تبلور فاز فسفات کلسیم با عبور از دمای ˚C800 آغاز گردید. پیوندهای ایجادشده در ساختار نیز توسط طیف‌سنجی مادون قرمز تبدیل فوریه (FTIR) شناسایی شد. طبق تصویربرداری توسط میکروسکوپ AFM، زبری سطح ذرات فرآوری‌شده nm32/ 17 است. به‌منظور درک رفتار مکانیکی و ریزساختاری داربست‌ها آزمون خمش سه‌نقطه و تصویربرداری توسط میکروسکوپ الکترونی روبشی(FESEM) انجام شد. نتایج بررسی‌ها نشان داد که استحکام خمشی نهایی داربست برابر با MPa3/1±15 بوده و دارای ریزساختاری کاملاً متخلخل می‌باشد. مطالعه رفتار زیستی داربست توسط آنالیز Resazurin Red Assay نیز عدم سمیت، رشد و تکثیر مطلوب سلول‌های بنیادی مغز دندان عقل را در مجاورت با داربست اثبات نمود. همچنین قراردادن داربست‌ها در محلول شبیه‌سازی-شده بدن با رهایش مطلوب یون‌های Ca2+ ، Si4+وPO43- همراه بود. بنابراین، می‌توان ادعا نمود داربست‌های ژلاتین- فسفات‌کلسیم می‌توانند برای ترمیم بافت‌های آسیب‌دیده استخوانی مفید واقع شوند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Gelatin-Calcium Phosphate Composite Scaffold: From Fabrication to Mechanical and Biological Properties Investigation

نویسندگان [English]

  • Faezeh Darvishian Haghighi 1
  • Sahar Moaveni 2
  • sahar mollazadeh 3
  • samane sahebian 3
  • Zahra Tayarani Najaran 4
1 Department of Material Science and Engineering, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Pharmacological Research Center of Medicinal Plants, University of Medical Sciences, Mashhad, Iran
3 Department of Material Science and Engineering, Ferdowsi University of Mashhad, Mashhad, Iran.
4 Pharmacological Research Center of Medicinal Plants, University of Medical Sciences, Mashhad, Iran
چکیده [English]

The fabrication of the most similar structure to the bone with appropriate mechanical, physical, and biological characteristics is challenging. The main aim of the present research is to design, fabricate, and characterize the gelatin-calcium phosphate composite scaffolds. First, calcium phosphate particles (SCP) were synthesized through the sol-gel process. Accordingly, the prepared particles were synthesized via the sol-gel route and heat-treated at 1100℃. Then, the gelatin-calcium phosphate composite scaffolds were fabricated through solvent casting and freeze-drying methods. According to the pycnometer and particle size (PSA) analyses, the density and the particle size of the SCP particles were 4.06 g/cm3 and 37±5 nm, respectively. Based on the XRD and DTA-TG results of the particles, crystallization of the calcium phosphate phases has been started at 800℃. The functional groups of the particles have been also studied through FTIR analysis. The roughness of the particles was 17.32nm based on the AFM microscopy results. According to the three-point flexural test, the final strength of the scaffolds was 15 MPa. Field emission scanning electron microscopy (FESEM) showed that the scaffolds had a completely porous structure with interconnected pores. Resazurin Red Assay confirmed the viability of 78% of stem cells on the scaffolds after 5 days of seeding. Immersion of the scaffolds in the simulated body fluid (SBF) caused the controlled release of the Ca2+, Si4+, and PO43- ions. Altogether, the gelatin-calcium phosphate composite scaffolds have a promising role in tissue engineering applications.

کلیدواژه‌ها [English]

  • Bone Scaffold
  • Gelatin
  • Calcium phosphate
  • Stem Cell Culture
  • Mechanical and Biological Properties

مراجع

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